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| Figure 1. TSA plates after the solution of TSA powder and distilled water was poured. |
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| Figure 2. 14 TSA plates with unknown bacteria (0 hours) |
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| Figure 3. 14 TSA plates with unknown bacteria after 24 hours |
Thursday, August 31, 2017
Returning To S-STEM
I am grateful for having the opportunity to continue my S-STEM experience because it is a truly amazing place with wonderful people. During my first week, I had the chance to make my own TSA plates and also culture unknown bacteria. Most of the bacteria grew in 24 hours, others in 48 hours and about 2 showed no growth (currently growing at room temperature) (Figures 1-3). The purpose of this is to then be able to grow bacteria in a broth, perform a DNA extraction method and, eventually, be able to identify what kind of bacteria it is. All of this ties into this semester’s research project with WAESO, which I am very excited about! I will be working with isolated bacteria from leaves of a creosote bush (Larrea tridentata) and using genetic sequencing to identify the bacteria present. Fortunately, this will be a continuance of what I did last semester so I cannot wait to see what this research has in store!
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Hey, Brenda, I’m glad you got to continue your S-STEM program. I have only been here for a week, and I love it. I can only imagine how awesome it is to do it for a second consecutive semester. I too had the chance to culture my unknown bacteria but never got to do my own TSA plate. You were right; some bacteria’s only need about 48 hours to grow at room temperature (37oC). There are also specific bacteria’s like ‘mesophilic’ bacteria that require 40oC to grow. Maybe that’s one reason you didn’t get to see full growth. As for your WAESO research project, I didn’t quite get what you guys had planned. What did you mean by “Corossol leaves?”
ReplyDeleteI messed up. It sounded to me like "corossol leaves" but it's actually leaves from a creosote bush (Larrea tridentata). I edited the post so I hope that makes much more sense and sorry for the confusion!
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