Unknown Bacteria NanoDrop Data

Using the unknown extracted DNA samples from last week, I ran them in the NanoDrop and have included the results below. Figure 1 shows the amount of nucleic acid found in each unknown and it’s purity (the 260/280 ratio should be around 1.7 to be considered pure). From looking at the table, I was hoping to get higher amounts of DNA (above 600). Due to this, I will continue to perform the QuickExtract Bacterial DNA Extraction to yield higher quantities. Nonetheless, the samples remained around the 1.7 260/230 ratio, which I am happy about. In addition, Figure 2 shows the ideal dumbbell shape the wavelength of the samples produced.

Figure 1. The NanoDrop data collected from unknown bacteria in creosote bushes. Five DNA extracted samples were used: A1, A8, A24, A26 and A27. Displayed with each bacteria is the amount of nucleic acid, 260 ratio, 280 ratio, 260/280 ratio and 260/230 ratio.

Figure 2. The NanoDrop graph from unknown bacteria in creosote bushes. The five displayed DNA extracted samples, A1, A8, A24, A26 and A27, are shown, along with the wavelength and the absorbance.